6PK3
Alanine-glyoxylate aminotransferase 1 (AGT1) from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 139.179, 62.289, 97.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.600 - 2.180 |
R-factor | 0.1415 |
Rwork | 0.139 |
R-free | 0.17030 |
Structure solution method | MIR |
RMSD bond length | 0.003 |
RMSD bond angle | 0.542 |
Data reduction software | DENZO (0.95) |
Data scaling software | SCALEPACK (1.6.1) |
Phasing software | SOLVE (2.02) |
Refinement software | PHENIX (1.15-2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.600 | 30.000 | 2.280 |
High resolution limit [Å] | 2.180 | 4.740 | 2.200 |
Rmerge | 0.077 | 0.042 | 0.209 |
Number of reflections | 41368 | 4648 | 2127 |
<I/σ(I)> | 15.4 | ||
Completeness [%] | 92.1 | 97.3 | 48.2 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | Protein: 11 mg/ml in 0.2 mM pyridoxal-phosphate, 10% glycerol, 100 mM Tris-HCl, pH 8.5. Precipitant: 4.1-4.2 M sodium formate. One microliter protein + 1 microliter precipitant equilibrated against precipitant. |