6PK3

Alanine-glyoxylate aminotransferase 1 (AGT1) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2002-03-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	139.179, 62.289, 97.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.600 - 2.180
R-factor	0.1415
Rwork	0.139
R-free	0.17030
Structure solution method	MIR
RMSD bond length	0.003
RMSD bond angle	0.542
Data reduction software	DENZO (0.95)
Data scaling software	SCALEPACK (1.6.1)
Phasing software	SOLVE (2.02)
Refinement software	PHENIX (1.15-2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.600	30.000	2.280
High resolution limit [Å]	2.180	4.740	2.200
Rmerge	0.077	0.042	0.209
Number of reflections	41368	4648	2127
<I/σ(I)>	15.4
Completeness [%]	92.1	97.3	48.2
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	Protein: 11 mg/ml in 0.2 mM pyridoxal-phosphate, 10% glycerol, 100 mM Tris-HCl, pH 8.5. Precipitant: 4.1-4.2 M sodium formate. One microliter protein + 1 microliter precipitant equilibrated against precipitant.