6PIC
Crystal structure of Marinobacter subterrani acetylpolyamine amidohydrolase (msAPAH) complexed with 6-amino-N-hydroxyhexanamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.340, 67.040, 167.531 |
| Unit cell angles | 90.00, 90.00, 92.60 |
Refinement procedure
| Resolution | 51.973 - 2.031 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: 4ZUM |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.970 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rpim | 0.158 | 0.403 |
| Number of reflections | 154543 | 15944 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 85.9 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/ml msAPAH Protein, 0.2 M magnesium acetate tetrahydrate , 20% w/v PEG 3350, 1:1 ratio protein to precipitant |
