6PHC

Pfs25 in complex with the human transmission blocking antibody 2544

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 31 2 1
Unit cell lengths	141.220, 141.220, 164.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.568 - 2.900
R-factor	0.2022
Rwork	0.200
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.002
RMSD bond angle	0.582
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.236	0.832
Rpim	0.088	0.249
Number of reflections	42529	4085
<I/σ(I)>	9.9	1.4
Completeness [%]	99.9	100
Redundancy	9.9	10.4
CC(1/2)	0.993	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M HEPES NaOH, pH 7.5, 20 % (w/v) PEG 4000, 10 % (v/v) 2-propanol