6PFL
Crystal structure of Human HUWE1 WWE domain in complex with ADPR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 50.423, 50.423, 128.412 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.670 - 2.100 |
R-factor | 0.2106 |
Rwork | 0.208 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6miw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.439 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.670 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.074 | 0.528 |
Rmeas | 0.082 | 0.575 |
Rpim | 0.032 | 0.221 |
Total number of observations | 67269 | 5537 |
Number of reflections | 11221 | 904 |
<I/σ(I)> | 11.3 | 2.1 |
Completeness [%] | 97.8 | 99.5 |
Redundancy | 6 | 6.1 |
CC(1/2) | 0.997 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.4M NaCitrate, and 0.1M Tris-HCL pH7.5 |