6PD5

Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	108.056, 108.056, 419.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.350 - 2.390
R-factor	0.2156
Rwork	0.213
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fk0
RMSD bond length	0.009
RMSD bond angle	1.716
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.520
High resolution limit [Å]	2.390	2.390
Rmeas	0.163	1.764
Number of reflections	37825	5929
<I/σ(I)>	12.29	2.57
Completeness [%]	99.5	98.6
Redundancy	9.14	8.65
CC(1/2)	0.997	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM cacodylate, pH 6.5, 200 mM sodium chloride, 2 M ammonium sulfate, 10% glycerol