6PBR
Catalytic domain of E.coli dihydrolipoamide succinyltransferase in I4 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-19 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92 |
Spacegroup name | I 4 |
Unit cell lengths | 128.598, 128.598, 249.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.360 - 3.000 |
R-factor | 0.2324 |
Rwork | 0.230 |
R-free | 0.27172 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c4t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | SIMBAD (0.1.10) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.360 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.220 | 1.470 |
Rpim | 0.125 | 0.860 |
Number of reflections | 33570 | 5050 |
<I/σ(I)> | 3.7 | 0.8 |
Completeness [%] | 82.9 | 85.5 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.980 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 50 mM Tris pH 8.0 2.6 M NaCl |