6PBK
Archaellum periplasmic stator protein complex FlaF and FlaG from Sulfolobus acidocaldarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 61 |
Unit cell lengths | 120.038, 120.038, 152.138 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.292 - 2.805 |
R-factor | 0.1728 |
Rwork | 0.171 |
R-free | 0.20210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p94 5tuh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.097 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.190 | 49.190 | 2.960 |
High resolution limit [Å] | 2.800 | 8.870 | 2.800 |
Rmerge | 0.083 | 0.025 | 1.265 |
Rmeas | 0.090 | 0.027 | 1.370 |
Rpim | 0.034 | 0.010 | 0.521 |
Total number of observations | 206623 | 6445 | 30017 |
Number of reflections | 30449 | 992 | 4409 |
<I/σ(I)> | 16.4 | 56.3 | 1.5 |
Completeness [%] | 99.9 | 99.2 | 99.7 |
Redundancy | 6.8 | 6.5 | 6.8 |
CC(1/2) | 0.999 | 1.000 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 18% PEG 6000, 0.1 M Tris, pH 7.5, and 0.2 M NaBr |