6PAM
Structure of a bacterial Atm1-family ABC transporter with MgADP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 129.180, 133.610, 134.260 |
| Unit cell angles | 110.62, 98.28, 101.20 |
Refinement procedure
| Resolution | 39.606 - 3.700 |
| R-factor | 0.2406 |
| Rwork | 0.238 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mrn |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.610 | 4.660 | 3.832 |
| High resolution limit [Å] | 3.700 | 4.500 | 3.700 |
| Rmerge | 0.167 | 3.241 | 3.050 |
| Rmeas | 0.180 | 3.320 | 3.283 |
| Rpim | 0.067 | 0.712 | 1.206 |
| Number of reflections | 84628 | 4667 | 7646 |
| <I/σ(I)> | 7.31 | 1.4 | 0.74 |
| Completeness [%] | 97.5 | 99.3 | 89.28 |
| Redundancy | 7.1 | 21.4 | 7.3 |
| CC(1/2) | 0.999 | 0.761 | 0.412 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 296 | NaCl, MgCl2, Tris, PEG2000MME, ATP |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 296 | NaCl, MgCl2, Tris, PEG2000MME, ATP |
