6P98
OXA-48 carbapanemase, meropenem complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.132, 74.563, 97.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.566 - 1.750 |
| R-factor | 0.1902 |
| Rwork | 0.189 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 53334 | 2896 |
| <I/σ(I)> | 13.3 | 1.9 |
| Completeness [%] | 97.8 | |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 mM Ca2+, 5 mM Co2+, 5 mM Cd2+, 20% PEG 3350 |
