6P74
OLD nuclease from Thermus Scotoductus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0718 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 83.362, 101.737, 202.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.377 - 2.200 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.22910 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.380 | 2.180 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 49235 | 741 |
<I/σ(I)> | 17.6 | |
Completeness [%] | 100.0 | |
Redundancy | 18.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M NaOAc pH 5.8, 0.3 M AmSO4, 7% PEG MME 2000, and 5 mM SmCl3 |