6P70
X-ray crystal structure of bacterial RNA polymerase and pyrBI promoter complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.179, 100.845, 294.865 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 46.061 - 3.052 |
R-factor | 0.2083 |
Rwork | 0.207 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vo8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.451 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.130 |
High resolution limit [Å] | 3.050 | 3.050 |
Rpim | 0.068 | 0.549 |
Number of reflections | 101835 | 4930 |
<I/σ(I)> | 10.9 | 1.21 |
Completeness [%] | 99.7 | 97 |
Redundancy | 3.4 | 2.9 |
CC(1/2) | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000 |