6P66
The crystal structure of the XPB complex with Bax1 from Archaeoglobus fulgidus at 3.0 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 200.600, 129.840, 108.150 |
Unit cell angles | 90.00, 104.30, 90.00 |
Refinement procedure
Resolution | 39.932 - 3.000 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fwr |
Data reduction software | MOSFLM (7.2.0) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 107.969 | 39.932 | 3.160 |
High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
Rmerge | 0.034 | 0.670 | |
Rmeas | 0.090 | 0.042 | 0.908 |
Rpim | 0.048 | 0.022 | 0.472 |
Total number of observations | 182895 | 5885 | 27385 |
Number of reflections | 52884 | 1650 | 7664 |
<I/σ(I)> | 8.4 | 29.8 | 1.4 |
Completeness [%] | 98.5 | 94.1 | 98.4 |
Redundancy | 3.5 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1:1 ratio of protein to mother liquor (0.1 M sodium acetate pH 4.8, 1.8 M sodium acetate) |