6P4X

Crystal Structure of the S. cerevisiae glucokinase, Glk1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	177.750, 177.750, 323.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.830 - 3.590
R-factor	0.2685
Rwork	0.267
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ig8
RMSD bond length	0.005
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	155.830	3.718
High resolution limit [Å]	3.590	3.590
Rmerge	0.100	0.483
Rmeas	0.142	0.683
Rpim	0.100	0.483
Number of reflections	61332	6026
<I/σ(I)>	8.28	1.56
Completeness [%]	100.0	99.95
Redundancy	2	2
CC(1/2)	0.988	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293.15	2.4M Diammonium Phosphate, 0.1M CHES pH 9.4