6P4W

XPB helicase in a complex with truncated Bax1 from Sulfurisphaera tokodaii at 2.96 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	P 1
Unit cell lengths	56.415, 101.369, 114.476
Unit cell angles	83.09, 81.15, 90.17

Refinement procedure

Resolution	29.280 - 2.956
R-factor	0.1855
Rwork	0.183
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tnu
RMSD bond length	0.012
RMSD bond angle	1.593
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.098	0.053	0.822
Rmeas	0.116	0.062	0.982
Rpim	0.061	0.033	0.529
Total number of observations	179503
Number of reflections	51223	2569	2523
<I/σ(I)>	5.8
Completeness [%]	99.1	99.7	98.3
Redundancy	3.5	3.5	3.3
CC(1/2)		0.996	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	200 mM Ammonium citrate, pH 7.5, 8% PEG 3350