6P4F

Crystal structure of the XPB-Bax1-forked DNA ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7883
Spacegroup name	C 1 2 1
Unit cell lengths	214.687, 92.150, 172.128
Unit cell angles	90.00, 132.24, 90.00

Refinement procedure

Resolution	39.735 - 3.550
R-factor	0.2513
Rwork	0.250
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5TNU and 6P4W
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	127.432	39.735	3.740
High resolution limit [Å]	3.550	11.230	3.550
Rmerge		0.050	1.451
Rmeas	0.266	0.061	1.755
Rpim	0.148	0.034	0.973
Total number of observations	85678	2630	12512
Number of reflections	28493	867	4105
<I/σ(I)>	4.9	14.1	1.3
Completeness [%]	94.1	85.8	94.3
Redundancy	3	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	293	50mM MES, pH 5.3; 10mM MgCl2; 26% MPD