6P4A
HyHEL10 Fab complexed with hen egg lysozyme carrying two mutations (HEL2x-rigid): R21Q and R73E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.622, 55.245, 74.234 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.200 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.500 | 48.500 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.094 | 0.038 | 0.649 |
| Rmeas | 0.102 | 0.041 | 0.703 |
| Rpim | 0.039 | 0.015 | 0.267 |
| Total number of observations | 178665 | 2714 | 14648 |
| Number of reflections | 26223 | 415 | 2181 |
| <I/σ(I)> | 11.9 | 37.6 | 2.7 |
| Completeness [%] | 99.4 | 99.6 | 97 |
| Redundancy | 6.8 | 6.5 | 6.7 |
| CC(1/2) | 0.998 | 0.999 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Bis-Tris propane, pH 7.0, 22% PEG3350 |
