6P2U

Structure of Mortalin-NBD with N6-propargyladenosine-5'-diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-08-30
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	51.040, 68.250, 121.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 2.000
R-factor	0.2171
Rwork	0.215
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kbo
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.040	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	29169	2845
<I/σ(I)>	10.62
Completeness [%]	99.3	98.44
Redundancy	6.9
CC(1/2)	0.994	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	30% w/v PEG8000, 100 mM sodium acetate, 200 mM lithium sulfate