6P1W

Pre-catalytic ternary complex of human DNA Polymerase Mu with 1-nt gapped substrate containing undamaged template dG and bound incoming CMPCPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-24
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.802, 68.692, 110.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.901 - 1.751
R-factor	0.1645
Rwork	0.163
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.076
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmeas	0.059	0.456
Rpim	0.017	0.207
Number of reflections	46387	2227
<I/σ(I)>	34.4	3.13
Completeness [%]	99.9	97.9
Redundancy	10.1	4.5
CC(1/2)		0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	82mM HEPES pH 7.5, 82mM NaCl, 8.2% w/v PEG4000