6P1U

Post-catalytic nicked complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and newly incorporated CMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-13
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.864, 68.825, 110.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.982 - 1.750
R-factor	0.1704
Rwork	0.169
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.118
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmeas	0.066	0.623
Rpim	0.019	0.270
Number of reflections	46921	2265
<I/σ(I)>	30.1	2.84
Completeness [%]	100.0	99.9
Redundancy	8.3	5.2
CC(1/2)		0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	94mM HEPES pH 7.5, 94mM NaCl, 9.4% v/s PEG4000