6P1T
Pre-catalytic ternary complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and bound CMPCPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-10 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.805, 68.715, 110.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.042 - 1.700 |
R-factor | 0.1702 |
Rwork | 0.169 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.056 | 0.633 |
Rpim | 0.016 | 0.317 |
Number of reflections | 49662 | 1927 |
<I/σ(I)> | 31.6 | 2.09 |
Completeness [%] | 97.6 | 77.5 |
Redundancy | 8.5 | 3.2 |
CC(1/2) | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 94mM HEPES pH 7.5, 94mM NaCl, 9.4% w/v PEG4000 |