6P1R

Pre-catalytic ternary complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and bound incoming AMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-26
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.889, 68.770, 110.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.372 - 1.701
R-factor	0.1753
Rwork	0.174
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.101
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmeas	0.051	0.436
Rpim	0.015	0.184
Number of reflections	50827	2452
<I/σ(I)>	40.6	2.96
Completeness [%]	99.8	98.4
Redundancy	7.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	100mM HEPES pH 7.5, 100mM NaCl, 10% w/v PEG4000