6P1Q
Post-catalytic nicked complex of human DNA Polymerase Mu with 1-nt gapped substrate containing template 8OG and newly incorporated dCMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-06 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.768, 68.740, 110.479 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.072 - 1.900 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.20640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.102 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.063 | 0.486 |
Rpim | 0.018 | 0.221 |
Number of reflections | 36686 | 1768 |
<I/σ(I)> | 37.8 | 2.8 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 10.3 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100mM HEPES pH 7.5, 100mM NaCl, 10% w/v PEG4000 |