6P1L
Crystal structure of EGFR in complex with EAI045
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.600, 103.150, 87.100 |
Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
Resolution | 58.609 - 2.800 |
R-factor | 0.2027 |
Rwork | 0.201 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d41 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.610 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.116 | 0.673 |
Rmeas | 0.139 | 0.819 |
Rpim | 0.076 | 0.459 |
Total number of observations | 91347 | 6038 |
Number of reflections | 30003 | 2141 |
<I/σ(I)> | 8.4 | 1.9 |
Completeness [%] | 96.5 | 93.5 |
Redundancy | 3 | 2.8 |
CC(1/2) | 0.991 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris ,26% PEG 8000 |