6OZ4
Crystal structure of broadly neutralizing antibody N49P6 Fab in complex with HIV-1 BG505 SOSIP.664 Env trimer ectodomain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 3 |
Unit cell lengths | 164.703, 164.703, 164.703 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.946 - 4.050 |
R-factor | 0.2578 |
Rwork | 0.255 |
R-free | 0.31460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvp 6bck |
RMSD bond length | 0.008 |
RMSD bond angle | 1.216 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.120 |
High resolution limit [Å] | 4.050 | 4.050 |
Rmerge | 0.113 | |
Rpim | 0.053 | 0.757 |
Number of reflections | 12543 | 608 |
<I/σ(I)> | 22.8 | 1 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 5.5 | 4.6 |
CC(1/2) | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 12% PEG 3350 0.1 M Tris-HCl pH 8.0 |