6OYL
The structure of the PP2A B56 subunit KIF4A complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.290, 108.037, 117.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.518 - 3.150 |
R-factor | 0.2162 |
Rwork | 0.215 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k6s |
RMSD bond length | 0.003 |
RMSD bond angle | 0.652 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.520 | 3.360 |
High resolution limit [Å] | 3.148 | 3.148 |
Rmerge | 0.105 | |
Rmeas | 0.113 | |
Number of reflections | 11932 | 1801 |
<I/σ(I)> | 11.5 | |
Completeness [%] | 96.6 | 83.1 |
Redundancy | 8.2 | 7.7 |
CC(1/2) | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 0.1 M HEPES, pH 7.75, 0.8 M lithium chloride, 8% PEG8000 |