6OX5
A SETD3 Mutant (N255A) in Complex with an Actin Peptide with His73 Replaced with Lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.465, 116.497, 174.655 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.938 - 2.098 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.20480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.632 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.940 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.158 | 1.022 |
Rpim | 0.036 | 0.450 |
Number of reflections | 35098 | 2968 |
<I/σ(I)> | 18.5 | 2.2 |
Completeness [%] | 96.6 | 82.9 |
Redundancy | 16.1 | 5.5 |
CC(1/2) | 0.384 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |