6OX2
SETD3in Complex with an Actin Peptide with the Target Histidine Fully Methylated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.098, 175.941, 66.264 |
Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
Resolution | 43.560 - 2.089 |
R-factor | 0.2002 |
Rwork | 0.199 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.427 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.570 | 2.160 |
High resolution limit [Å] | 2.089 | 2.089 |
Rmerge | 0.142 | 0.911 |
Rpim | 0.060 | 0.443 |
Number of reflections | 78578 | 7372 |
<I/σ(I)> | 12.6 | 1.6 |
Completeness [%] | 97.0 | 91.5 |
Redundancy | 6.1 | |
CC(1/2) | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |