6OU0

Crystal Structure of the D380A/D478S Variant of the Myocilin Olfactomedin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2018-07-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.155, 56.931, 90.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.378 - 1.799
R-factor	0.1633
Rwork	0.161
R-free	0.18880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.659
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.380	1.860
High resolution limit [Å]	1.799	1.799
Rmerge	0.093
Number of reflections	22208	2164
<I/σ(I)>	18.67	4.27
Completeness [%]	99.7
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	15% PEG 3350, 0.2 M Bis Tris pH 6, 0.2 M Magnesium formate