6OSV

Potent and Selective Antitumor Antibody Targeting a Membrane-Proximal Epitope of ROR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.109, 60.105, 102.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.378 - 1.340
R-factor	0.1625
Rwork	0.162
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.881	1.406
High resolution limit [Å]	1.340	1.340
Rmerge	0.033	0.678
Rmeas	0.036	0.795
Rpim	0.014	0.409
Number of reflections	55957	2799
<I/σ(I)>	22	1.23
Completeness [%]	91.5
Redundancy	5.9
CC(1/2)	1.000	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.01 M Magnesium chloride hexahydrate, 0.005 M Nickel(II) chloride 0.1 M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3,350