6OS5
Crystal structure of CymD prenyltransferase complexed with L-tryptophan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.330, 86.330, 141.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.665 - 1.660 |
R-factor | 0.1633 |
Rwork | 0.163 |
R-free | 0.19230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6os6 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.330 | 86.330 | 1.690 |
High resolution limit [Å] | 1.660 | 9.090 | 1.660 |
Rmerge | 0.112 | 0.187 | 0.398 |
Rmeas | 0.123 | 0.204 | 0.440 |
Rpim | 0.051 | 0.080 | 0.184 |
Total number of observations | 681939 | 5417 | 34015 |
Number of reflections | 123383 | 882 | 6056 |
<I/σ(I)> | 8.9 | 15.6 | 3.5 |
Completeness [%] | 99.6 | 99.3 | 99.7 |
Redundancy | 5.5 | 6.1 | 5.6 |
CC(1/2) | 0.986 | 0.957 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | (0.04 M citric acid, 0.06 M bis-tris propane (pH 6.4)), 20% PEG 3350 |