6OR0
Crystal structure of Insulin from Non-merohedrally twinned crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER TURBO X-RAY SOURCE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-25 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.031, 78.031, 78.031 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.180 - 1.550 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.990 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELXDE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.180 | 1.570 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Total number of observations | 22188 | |
| Number of reflections | 11668 | 459 |
| <I/σ(I)> | 62.79 | 2.23 |
| Completeness [%] | 98.4 | 70.8 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 0.2 M Na2HPO4/Na3PO4, pH 10.0 and 0.01 M Na EDTA |
