6OPG
phosphorylated ERK2 with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.850, 77.150, 152.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.890 - 2.900 |
| R-factor | 0.16039 |
| Rwork | 0.156 |
| R-free | 0.23907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.673 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.107 | 0.316 |
| Rmeas | 0.342 | |
| Rpim | 0.129 | |
| Number of reflections | 11508 | 1636 |
| <I/σ(I)> | 6.6 | 2.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 36% PEG3350, 0.1 M Tris pH 8.8 10mg/mL protein. |
