6OMS
Arabidopsis GH3.12 with Chorismate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.438, 67.002, 100.964 |
Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
Resolution | 48.589 - 1.942 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eql |
RMSD bond length | 0.007 |
RMSD bond angle | 0.810 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 85974 | 0 |
<I/σ(I)> | 8.9 | 1.8 |
Completeness [%] | 98.7 | 94.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG-3350, 0.25 M ammonium acetate, 100 mM sodium acetate pH 4.4, 5 mM tris(2-carboxyethyl)phosphine, 5 mM MgCl2, 10 mM chorismate, and 4 mM AMP |