6OMK

Crystal structure of a glycylpeptide N-tetradecanoyltransferase (N-myristoyl transferase, NMT) from Leishmania major Friedlin bound to tetradecanoyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5406
Spacegroup name	P 21 21 21
Unit cell lengths	49.250, 89.170, 90.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.585 - 2.100
R-factor	0.1748
Rwork	0.170
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h5z
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.15_3459))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.585	44.585	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.103	0.058	0.552
Rmeas	0.112	0.063	0.605
Total number of observations	164416
Number of reflections	23914	314	1756
<I/σ(I)>	12.54	28.97	3.5
Completeness [%]	100.0	98.4	100
Redundancy	6.875	5.879	6.032
CC(1/2)	0.997	0.996	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	287	LemaA.18219.a.B1.PS38467 at 3.52 mg/mL with 0.5 mM myristoyl CoA (tetradecanoyl CoA) against 16% PEG 3350, 0.2 M NaCl, 0.1 M sodium cacodylate pH 5.6, supplemented with 20% ethylene glycol as cryo-protectant, crystal tracking ID 303026d4, unique puck ID vle8-4