6OL7

Crystal structure of glVRC01 scFv in complex with anti-idiotype iv8 scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	51.583, 107.937, 116.961
Unit cell angles	85.49, 80.28, 84.17

Refinement procedure

Resolution	49.784 - 2.419
R-factor	0.1767
Rwork	0.174
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.506
High resolution limit [Å]	2.419	2.419
Number of reflections	87182	5909
<I/σ(I)>	10.25
Completeness [%]	92.4
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.13M NaCl, 0.13M Tris pH 8.0, 10.4% PEG 20,000