6OKQ
Crystal structure of the SF12 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 223.018, 223.018, 288.296 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.250 - 3.200 |
| R-factor | 0.275 |
| Rwork | 0.273 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.280 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.216 | 2.550 |
| Number of reflections | 70865 | 4473 |
| <I/σ(I)> | 22.8 | 1.4 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 60.4 | 56.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, pH 7.5, 1.8 M sodium formate |
