6OJW

Crystal structure of Sphingomonas paucimobilis TMY1009 holo-LsdA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12708
Spacegroup name	P 32 2 1
Unit cell lengths	183.259, 183.259, 95.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.353 - 2.600
R-factor	0.1887
Rwork	0.187
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.8)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.630	91.630	2.680
High resolution limit [Å]	2.600	11.030	2.600
Rmerge	0.133	0.032	1.007
Rmeas	0.141	0.034	1.074
Rpim	0.046	0.011	0.369
Number of reflections	56656	819	4597
<I/σ(I)>	15.4
Completeness [%]	99.9	99.9	99.7
Redundancy	9.2	9.9	8.4
CC(1/2)	0.996	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium acetate, 25% PEG3350 (v/v)