6OJF

Dimeric structure of LRRK2 GTPase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	44.634, 101.883, 44.610
Unit cell angles	90.00, 100.95, 90.00

Refinement procedure

Resolution	43.820 - 1.600
R-factor	0.1435
Rwork	0.143
R-free	0.15760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dpu
RMSD bond length	0.002
RMSD bond angle	1.295
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.590	4.340	1.600
Rmerge	0.084	0.058	0.577
Rmeas	0.087	0.060	0.623
Rpim	0.023	0.016	0.223
Number of reflections	49223	2668	1699
<I/σ(I)>	6.3
Completeness [%]	94.7	99.9	67.3
Redundancy	12.6	14.2	6
CC(1/2)		0.998	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	100mM KSCN, 25% PEGMME 2000, 0.1M BisTris pH 6.5