6OJE
Dimeric structure of LRRK2 GTPase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.647, 103.688, 44.593 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 43.800 - 1.950 |
| R-factor | 0.1623 |
| Rwork | 0.160 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RocCOR domain (3DPU) |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.072 | 0.049 | 0.457 |
| Rmeas | 0.078 | 0.053 | 0.516 |
| Rpim | 0.031 | 0.021 | 0.233 |
| Total number of observations | 189486 | ||
| Number of reflections | 31247 | 1617 | 1453 |
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 98.7 | 99.8 | 91.7 |
| Redundancy | 6.1 | 6.2 | 4.3 |
| CC(1/2) | 0.997 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 298 | 100mM KSCN, 25% PEGMME 2000, 0.1M BisTris pH 6.5 |
