6OIQ
Crystal structure of MYST acetyltransferase domain in complex with inhibitor 63
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.603, 56.772, 122.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 122.280 - 1.750 |
| R-factor | 0.18621 |
| Rwork | 0.184 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.280 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.055 | 0.197 |
| Number of reflections | 33519 | 3272 |
| <I/σ(I)> | 18.5 | 9.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 20% PEG 3350, 2% Tacsimate pH 7.0, 0.1 M HEPES pH 7.0 |
