6OIB
Crystal structure of human Sulfide Quinone Oxidoreductase in complex with coenzyme Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12713 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.516, 111.512, 133.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.370 - 2.030 |
R-factor | 0.2099 |
Rwork | 0.209 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.460 | 2.080 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.184 | 1.664 |
Rpim | 0.079 | 0.798 |
Number of reflections | 73848 | 4449 |
<I/σ(I)> | 6 | |
Completeness [%] | 96.9 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M ammonium tartrate dibasic, 20% PEG 3350 |