6OI2

Crystal structure of human WDR5 in complex with symmetric dimethyl-L-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.97931
Spacegroup name	P 21 21 2
Unit cell lengths	81.053, 86.285, 40.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.080 - 1.680
R-factor	0.18335
Rwork	0.181
R-free	0.22285
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h14
RMSD bond length	0.010
RMSD bond angle	1.435
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.080
High resolution limit [Å]	1.680
Rpim	0.030	0.420
Number of reflections	33411
<I/σ(I)>	15.7
Completeness [%]	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	295	100 mM Bis-Tris pH5.9, 32% PEG3350, 54.6 mM Ammonium Sulfate, 0.66 mM symmetric dimethyl-L-arginine