6OHO

Structure of human Phospholipase D2 catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	91.705, 131.336, 107.294
Unit cell angles	90.00, 111.77, 90.00

Refinement procedure

Resolution	48.570 - 2.000
R-factor	0.1835
Rwork	0.181
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ohm
RMSD bond length	0.005
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	79191	7826
<I/σ(I)>	6.3
Completeness [%]	99.0
Redundancy	6.6
CC(1/2)	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	298	0.2M ammonium sulfate, 20% PEG3350, 0.1M bis-tris pH 6.7