6OHH

Structure of EF1p2_mFAP2b bound to DFHBI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-13
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	36.350, 35.564, 86.596
Unit cell angles	90.00, 90.68, 90.00

Refinement procedure

Resolution	25.420 - 2.100
R-factor	0.1725
Rwork	0.169
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.859
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.140
High resolution limit [Å]	2.100	5.690	2.100
Rmerge	0.025	0.020	0.055
Rmeas	0.030	0.023	0.068
Rpim	0.016	0.012	0.040
Number of reflections	13078	706	570
<I/σ(I)>	16.6
Completeness [%]	98.9	99.4	86.2
Redundancy	3.6	3.5	2.8
CC(1/2)		1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M HEPES pH 7.5, 25% PEG3350