6OH4
X-ray crystal structure of the mouse CMP-sialic acid transporter in complex with CMP, by hanging drop vapor diffusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.820, 193.960, 66.440 |
| Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.380 |
| R-factor | 0.29094 |
| Rwork | 0.289 |
| R-free | 0.32106 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.500 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Number of reflections | 11456 | 51 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 67.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 25.6-26.8% PEG 400, 0.1 M Tris pH 8.5, and 0.1 M magnesium acetate |
