6OE5

Splayed open prefusion RSV F captured by CR9501 and motavizumab Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 61
Unit cell lengths	219.837, 219.837, 68.811
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.677 - 4.100
R-factor	0.2637
Rwork	0.262
R-free	0.30570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.501
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.680	43.680	4.580
High resolution limit [Å]	4.100	9.170	4.100
Rmerge	0.295	0.070	1.267
Rmeas	0.318	0.076	1.366
Rpim	0.116	0.028	0.505
Total number of observations	110763
Number of reflections	15219	1412	4299
<I/σ(I)>	5.3
Completeness [%]	99.9	98.8	100
Redundancy	7.3	7	7.1
CC(1/2)	0.988	0.988	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4.69 mg/mL prefusion F (PR-DM) + motavizumab Fab + CR9501 Fab, 30% (v/v) PEG400, 0.19 M ammonium sulfate, 3.1% (w/v) PEG8000, 0.1 M Tris pH 8.5