6OD3

Human TCF4 C-terminal bHLH domain in Complex with 13-bp Oligonucleotide Containing E-box Sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	47.180, 58.946, 62.720
Unit cell angles	104.63, 90.34, 94.86

Refinement procedure

Resolution	37.904 - 1.494
R-factor	0.2218
Rwork	0.221
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ql2
RMSD bond length	0.002
RMSD bond angle	0.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.904	1.540
High resolution limit [Å]	1.490	1.490
Rmerge	0.056	0.730
Rpim	0.033	0.471
Number of reflections	169064	12726
<I/σ(I)>	21.6	1.6
Completeness [%]	80.3	60.5
Redundancy	1.9
CC(1/2)		0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	25% PEG3350, 0.1 M Tris, pH 8.5, 0.2 M sodium chloride