6OCB

Crystal structure of FluA-20 Fab in complex with the head domain of H3 (A/Hong Kong/1/1968)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	84.554, 84.554, 271.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.814 - 2.100
R-factor	0.2039
Rwork	0.202
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fp8 6obz
RMSD bond length	0.014
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.040	0.330
Number of reflections	59126	2895
<I/σ(I)>	18.4	1.8
Completeness [%]	99.6	99.6
Redundancy	6.6	5.6
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M Tris-HCl pH 8.5 0.2 M lithium sulfate 40% (v/v) PEG400