6OBH
Structure of HIV-1 CA 1/2-hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.770, 77.746, 77.758 |
| Unit cell angles | 70.65, 70.70, 70.59 |
Refinement procedure
| Resolution | 45.000 - 2.960 |
| R-factor | 0.21099 |
| Rwork | 0.208 |
| R-free | 0.26469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.437 |
| Data reduction software | HKL-2000 (v715) |
| Data scaling software | HKL-2000 (v715) |
| Phasing software | PHASER (7.0.0) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.010 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.050 | |
| Number of reflections | 30532 | 2873 |
| <I/σ(I)> | 14.9 | 0.9 |
| Completeness [%] | 92.5 | 86.03 |
| Redundancy | 1.8 | |
| CC(1/2) | 0.351 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 293 | 0.1 M PCB buffer, pH 8 (sodium propionate, sodium cacodylate, Bis-Tris propane), 25% w/v PEG1500 |
