6OAL

Structure of human PARG complexed with JA2120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.302, 89.053, 94.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.964 - 1.600
R-factor	0.1825
Rwork	0.181
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.010
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.964	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.118	3.582
Number of reflections	74163	7334
<I/σ(I)>	18.59
Completeness [%]	100.0
Redundancy	19.8
CC(1/2)	1.000	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M (NH3)2SO4, 17% PEG3350